Prof.
Peter B. O'Connor's note on mass spectrometry. It deals with the interpretation
of mass spectra; it would be particularly useful for any persons undertaking the
analysis of unknown samples. It starts by dealing with a range of techniques,
among them being electron impact ionization, MS/MS fragmentation, and ion
sources. In addition, the note goes into practical implementations of mass
spectrometry in real-world applications such as protein complex analysis,
hydrogen and deuterium exchange, and imaging mass spectrometry. The course takes
the student through advanced topics like 2D FTICR mass spectrometry, an
expansive full view of modern mass spectrometric methods and their practical use
in research and industry.
This book serves as an introduction to
tandem mass spectrometry (MS/MS) and its role in molecular characterization in
chemical and biological research areas. It is tailored to professionals and
students alike and assesses the application of MS/MS in studying complex
molecules such as drugs, metabolites, carbohydrates, and proteins. The authors
explain how tandem mass spectrometry can provide detailed structural information
and insights into post-translational modifications. This resource is ideal for
those looking to understand the powerful applications of MS/MS in molecular
biology and chemistry, with emphasis on its versatility in biological and
pharmaceutical sciences.
Author(s): Ana Varela Coelho
and Catarina de Matos Ferraz Franco
This note presents
an overview of mass spectrometry at an advanced level with specific emphasis on
its utility in chemistry and biochemistry. Fundamental concepts include
ionization, mass analyzers, and dissociation of ions, as well as more advanced
topics like ion mobility and fragmentation. The qualitative and quantitative
analysis using mass spectrometry and the practical applications in various
fields are also considered. This note is designed for advanced students and
professionals aiming to gain insights into the role of mass spectrometry as an
analytical tool, both in theory and practice.